Journal: Cancer Cell International

Article Title: HOPX is a tumor-suppressive biomarker that corresponds to T cell infiltration in skin cutaneous melanoma

doi: 10.1186/s12935-023-02962-2

Figure Lengend Snippet: HOPX inhibits proliferation, migration, and invasion and induces apoptosis and cycle arrest in SKCM cell lines. ( A ) The overexpression of HOPX in A375 cells was confirmed by RT‒qPCR and Western blotting ( B ). ( C ) A CCK-8 assay was used to detect the proliferation of A375 cells. ( D ) A wound healing assay was used to detect the migration of A375 cells. ( E - H ) Transwell assays were used to detect the migration/invasion of A375 cells. ( I - J ) The apoptosis and cell cycle ( K - L ) of A375 cells were detected by flow cytometry

Article Snippet: In addition, the FastKing One Step RT‒qPCR Kit (SYBR) (TIANGEN, China) and an Eppendorf Quantitative PCR Instrument (Eppendorf, China) were used for all RT-qPCRs.

Techniques: Migration, Over Expression, Western Blot, CCK-8 Assay, Wound Healing Assay, Flow Cytometry

Journal: Molecular cell

Article Title: HOTTIP -dependent R-loop formation regulates CTCF boundary activity and TAD integrity in leukemia

doi: 10.1016/j.molcel.2022.01.014

Figure Lengend Snippet: Key resources table

Article Snippet: SingleShotTM SYBR® Green One-Step Kit , Bio-Rad Laboratories , Cat#1725095.

Techniques: Control, Recombinant, CRISPR, Electroporation, Protease Inhibitor, Staining, Transfection, Reverse Transcription, Purification, Plasmid Preparation, DNA Library Preparation, Multiplex Assay, Hybridization, Library Quantification, Sensitive Assay, SYBR Green Assay, Transgenic Assay, Software